2014细胞的传代培养实验.pptxVIP

GFP转染哺乳动物细胞实验 和细胞的传代培养实验 ;实验课流程安排;关于转染（transfection）;Transfection is the process of deliberately introducing nucleic acids into cells. The term is used notably for non-viral methods in eukaryotic cells. Genetic material (such as supercoiled plasmid DNA or siRNA constructs), or even proteins such as antibodies, may be transfected. Transfection of animal cells typically involves opening transient pores or holes in the cell membrane, to allow the uptake of material. DNA小片段插入体细胞或细胞系的过程，指真核细胞由于外源DNA掺入而获得新的遗传标??的过程 若此DNA未与宿主细胞DNA整合而获表达，称“瞬时转染(transient transfection)” 若与宿主细胞DNA整合并随后者的复制而复制称“稳定