雌激素对穹隆海马伞切断大鼠脑内nAchR的干预作用.doc

雌激素对穹隆海马伞切断大鼠脑内nAchR的干预作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：雌激素对穹隆海马伞切断大鼠脑内nAchR的干预作用 1 1 材料和方法 3 1．1 实验动物和分组 3 1．2 实验方法 3 121 模型制作 3 122 动物灌注、固定、取材 3 123 免疫组织化学染色检测（SABC法） 4 1．3 雌激素补充疗法[7] 4 1．4 实验数据与统计处理 4 2 结果 5 3 讨论 5 文2：葛根素对大鼠心肌缺血再灌注损伤的干预作用 7 1 材料与方法 8 2 结 果 10 3 讨 论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 雌激素对穹隆海马伞切断大鼠脑内nAchR的干预作用 文1：雌激素对穹隆海马伞切断大鼠脑内nAchR的干预作用 Abstract： Objective To observe the expression of nicotinic acetylcholine receptor (nAchR) in the brains fimbria/fornix traected rats, the effect of ectogenic estrogen on it, and its relation with Alzhelmers disease (AD). Methods Healthy female Wistar rats were divided into three groups at random: (1) fimbria/fornix traection group, (2) estrogen treatment group, and (3) control group. There were five rats in every group. And their fimbria/fornix was traected in order that AD models were made. Immunoreactive cells with nAchR were stained by immunohistochemical way so that the expression varieties of nAchR immunoreactive cells could be observed in hippocampus CA1 and cortex. Results In fimbria/fornix traection group, the expression of nAchR obviously decreased in the observed areas of the brain (P ). In estrogen treatment group, the expression of nAchR increased remarkably (P ). Conclusion The cells expressing nAchR clearly decrease in the areas of CA1 and cortex of rats when their fimbria/fornix is traected, but the cell number increases significanly if ectogenic estrogen is added. The change in nAchR is closely relative with AD whose pathological coue can be influenced by the intervention of estrogen. Key words： fimbriafornix; traection; nicotinic acetylcholine receptor; estrogen; Alzhelmers disease 阿尔茨海默病（Alzheimers Disease，AD）是一种以认知记忆障碍为主要特征的进行性神经系统疾病。其特征性病理改变为老年斑，神经元纤维缠结及神经元丢失以及多种神经递质和受体的改变。AD是神经系统进行性退行性疾病，其病因可能是多源性的，其中，乙酰胆碱受体与AD发病有密切的关系[1]。研究表明，雌激素与AD有密切关系[2]。本实验利用穹隆海马伞切断的方法模拟制作AD大鼠模型，观察AD发生时和给予外源性雌激素（E2）时，大鼠脑内海马CA1区和皮层区烟碱型乙酰胆碱受体nAc