当归多糖对HBV转基因小鼠树突状细胞作用.docVIP

当归多糖对HBV转基因小鼠树突状细胞作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：当归多糖对HBV转基因小鼠树突状细胞作用 1 1 材料与方法 3 2 结果与讨论 6 文2：C基因型HBV转基因小鼠的制备 8 0 引言 9 1 材料和方法 10 2 结果 11 1: 阳性对照; 2: 阴性对照. 12 1：阳性对照；2：阴性对照;M: marker. 12 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 当归多糖对HBV转基因小鼠树突状细胞作用 文1：当归多糖对HBV转基因小鼠树突状细胞作用 【Abstract】 Objective To observe the effect of Angelica sineis polysaccharide (ASP) on the function of dendritic cells(DCs) in HBV tragenic mice. Methods ASP of 1% concentration was given to HBV tragenic mice by abdominal injection twice a week. After 2 weeks, splee were harvested to make lymphocytes. MTT method was used to detect ASP’s cytotoxicity. Dendritic cells were separated from lymphocytes and incubated with granulocyte-macrophage colony-stimulating factor （GMC-SF）and interleukin-4（IL-4）. Phenotype of DCs were assayed by flowcytometry(FCM), IL-12 released by DCs and IL-10 and IFN-γ produced by T cells in mixed lymphocyte reaction(MLR) were measured by ELISA. Results ASP within the concentratio of 0-625mg/L didn’t show cytotoxicity to lymphocytes, ASP could enhance DCs’ secretion, increase IL-12 level in HBV tragenic mice , increase the secretion of IFN-γ, inhibit the secretion of IL-10 in MLR, thus up-regulates DC’s function. Conclusion ASP could up-regulate dendritic cells function in HBV tragenic mice that might be one mechanism through which polysaccharide regulate immune function. Our experiment results provided experimental evidence for the use of ASP in CHB clinic. 【Key words】 Angelica sineis polysaccharide (ASP); HBV tragenic mice; dendritic cell; chronic hepatitis B 慢性乙型肝炎(chronic hepatitis B, CHB)患者免疫功能低下，不能有效清除乙型肝炎病毒（HBV），机体对HBV呈现免疫耐受状态［1］。树突状细胞（dendritic cell, DC）是已知人体内功能最强大的抗原提呈细胞（antigen presenting cells, APC），在机体抗感染免疫中发挥重要作用。近年研究表明，CHB患者DC功能处于抑制状态，如何解决DC功能的低下状态成为国内外研究的热点，多糖类中药如香菇多糖等已被公认具有较好的免疫调节作用并广泛用于CHB和肿瘤的辅助 治疗 ［2］，鉴于多糖类中药良好的免疫调节作用，理论上推测其对DC应有一定的调节作用，但迄今 为止有关多糖对DC功能影响的报道尚不多见，因此，开展多糖类中药对DC影响的研究不仅开拓了多糖类研究的新领域，也有望为解决DC功