参芪扶正注射液对肝癌小鼠5FU化疗后免疫功能的影响临床医学.docVIP

参芪扶正注射液对肝癌小鼠5FU化疗后免疫功能的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：参芪扶正注射液对肝癌小鼠5FU化疗后免疫功能的影响临床医学 1 1 资料与方法 2 2 结果 4 3 讨论 5 文2：参芪扶正注射液对慢性阻塞性肺疾病患者免疫功能的影响 6 1 资料和方法 6 2 结果 7 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 11 正文 参芪扶正注射液对肝癌小鼠5FU化疗后免疫功能的影响临床医学 文1：参芪扶正注射液对肝癌小鼠5FU化疗后免疫功能的影响临床医学 Abstract Objective:To investigate the effect of Shenqi Fuzheng Injection on immune function of liver cancer mice after chemotherapy. Methods：Hepatoma 22 bearing mice models were liver cancer mice chemotherapy model was established by abdominal cavity injection of 5-Fu with a dose of 25mg/（kg·d) for five days. Then the mice were divided randomly into three groups, including control group and therapy (low and high dose) groups. Mice in therapy groups were given Shenqi Fuzheng Injection 40 mL/（kg·d) and 80 mL/（kg·d) respectively for 7 days. The control group were given saline 40 mL/（kg·d) for 7 days. After 24 h of the last treatment, the immune organ index was levels of IL-2 and TNF-a in mouse serum were measured By ：There were significant differences in the thymus index and spleen index between the treated groups and control group. It also significant increascd the level of IL-2 and decreased the level of TNF-a in the serum of the liver cancer mice after :Shenqi Fuzheng Injection can improve immune function in immunosuppressed mice after chemotherapy and be used clinically for the treatment of immunocompromised patients after chemotherapy. Key words Shenqi Fuzheng Injection;Liver cancer;Chemotherapy mice;Immune function 化疗是肝癌综合治疗的主要手段之一，但化疗药物在杀死肿瘤细胞的同时也对正常组织、细胞有一定程度的破坏作用。因此，如何降低化疗药物所致的细胞毒性和免疫功能低下是肝癌临床治疗面临的一大难题。参芪注射液是根据中医辨证论治的精湛理论而配方研制，以党参、黄芪为主药,而党参、黄芪为我国传统的扶正中药,可益气固本。本研究将参芪扶正注射液用于化疗后肝癌小鼠,以华内胸腺指数、脾脏指数、血清中IL-2和TNF-α水平变化为观察指标，探讨其对肝癌小鼠化疗后免疫功能的影响,为临床辅助肝癌治疗提供实验依据。 1 资料与方法 药品与试剂 5-FU由山西普德药业有限公司惠赠，参芪扶正注射液由丽珠集团惠赠，IL-2和TNF-α放免试剂盒购自北京东亚放射免疫研究所,其他试剂均为分析纯试剂。 动物 清洁级昆明小鼠36只，雌雄各半,6周龄～8周龄,SPF级，体重18 g～20 g，购自山西医科大学动物实验中心，许可证号(晋)2007-0002。 肿瘤细胞株 小鼠肝癌腹水株H22由山西医科大学药理教研室提供。 动物模