高铁血红素对体温过低症犬的保护作用研究.doc

高铁血红素对体温过低症犬的保护作用研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：高铁血红素对体温过低症犬的保护作用研究 1 1 材料和方法 2 2 结 果 3 3 讨 论 4 文2：加减清营汤对H2O2损伤的血管内皮细胞保护作用研究 5 1 实验材料 6 2 实验方法 7 3 结果 8 4 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 高铁血红素对体温过低症犬的保护作用研究 文1：高铁血红素对体温过低症犬的保护作用研究 Abstract: OBJECTIVE To explore the protective effect of hemin on hypothermic dogs immeed by seawater. METHODS Twelve healthy dogs were devided into two groups: control group and hemin hemin group, hemin (80 μmol/kg,twice a day)were administered through intraperitoneal injection for 2 days before hypothermia model was hemin was used to control immeed hyperthermia model was made in two groups. Dogs were immeed in cold seawater (12 ±2)℃ until the rectal temperature was decreased to 30℃. Plasma total bilirubin(TB),superoxide dismutase(SOD),aspartate aminotraferase (GOT),lactate dehydrogenase (LDH),creatine kinase (CK),CKMB,interleukin-6 (IL-6) and tumor necrosis factor (TNF-α)contents were measured before and after the model was the data were compared between two 　The content of TB and SOD of hemin group was higher than that of control group at 30℃(P＜).The content of GOT,LDH,CK,CKMB,IL-6 and TNF-α of hemin group was lower than that of control group at 30℃(P＜).CONCLUSION Hemin could exert protective effect on seawater immeed hypothermic dogs through elevating the serum level of SOD. Key words： Hypothermia;Superoxide dismutase;Total bilirubin;Hemin 体温过低症是指各种原因引起的中心温度低于35℃时的状态。体温过低会导致全身各系统的病理生理改变，当体温过低症状态持续较长时，体内的无氧代谢加剧，可导致乳酸血症。中、重度的体温过低症时死亡率极高。体温过低症的救治是急救医学和军事医学中的一项极其重要的课题。有研究表明血红素氧合酶（heme oxygenase，HO）参与炎性反应，具有抗氧化功能，是当前研究的热点［1］。本文研究血红素氧合酶诱导剂高铁血红素（hemin）在抗低温损伤中的作用，以期为体温过低症的救治提供新的思路。 1 材料和方法 材料 心电监护仪；Sar7400 血泵；普通酒精温度计；Shilley 温度监测仪及各种温度探头；Datascope 压力监测仪；Swan-Ganz导管；人工海水。高铁血红素（Sigma公司）用NaOH溶解后，磷酸盐缓冲液调整pH至，再用% NaCl稀释至 g/L作为贮备液。 动物及方法 健康杂种犬12 只，雌雄不限，体重（±）kg，由解放军总 医院 实验动物中心提供。随机分为对照组和高铁血红素组，每组6只。高铁红素组手术前48 h开始腹腔注射高铁血红素，每次80 μmol/kg，2次/天，连续2天。实验前犬禁食水，全身麻醉、气管插管，呼吸机辅助呼吸。连接心电图观察肢体导联 Ⅱ；连接皮温