抱茎蓼花的挥发油成分及其抗菌活性的研究.docVIP

抱茎蓼花的挥发油成分及其抗菌活性的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：抱茎蓼花的挥发油成分及其抗菌活性的研究 1 1 器材与方法 3 2 结果 5 3 结论 7 文2：大叶臭椒不同部位的挥发油成分及其抑菌活性分析 7 1 材料及方法 9 2 结果 10 3 讨论 11 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 抱茎蓼花的挥发油成分及其抗菌活性的研究 文1：抱茎蓼花的挥发油成分及其抗菌活性的研究 Abstract：ObjectiveTo extract and analyze the cotituents of the essential oil from the flower of Polygonum amplexicaule, so as to provide scientific proof for its exploitation of biological activity compositio. MethodsThe essential oil from the flower of Polygonum amplexicaule was extracted by steam distillation, the components of the essential oil were separated and structurally identified by gas chromatography/mass spectrometry, the relative contents of these components by the peak-area normalization method adopted in gas chromatography, and all components of the essential oil are calibrated with a standard mixture of homologous n-alkane series. The essential oil was investigated by antimicrobial activities. Results69 components were separated, 66 components were identified and accounted for % of the all peak area, the essential oil had antimicrobial activity. ConclusionThe main chemical cotituents of the essential oil are monoterpenoids and sesquiterpenoids compounds, the major components are Caryophyllen ( %), 3-Hexen-l-ol ( %), α-Linalool (%), 3-Octen-3-ol(%), etc. The essential oil has obvious inhibitory effect agait Escherichia coli, Salmonella typhi, Salmonella enteritidis and Staphylococcus aureus. Key words：Polygonum amplexicaule; Flower; Essential oil; Gas chromatography-mass spectrometry; Antimicrobial activity 陕西南部秦巴山区野生的抱茎蓼Polygonum amplexicaule 是蓼科Polynonaceae蓼属Polygonum L.的一种草本植物，又名红三七［1］。蓼属植物具有清热解毒、散结消肿、活血止痛、顺气解痉、收敛止泻等功效，有些还具有抗菌消炎、抗氧化、抗肿瘤、杀虫等多种生物活性［2］，因此对该属植物生物活性成分的研究较多［3］，对该属植物的挥发油也有一些研究报道［4］。抱茎蓼地上和地下部分的生物活性成分已有 文献 报道［5,6］，抱茎蓼叶子的挥发油也已有研究［1］，抱茎蓼花中的挥发性成分分析尚未见报道，挥发油有许多独特的生理活性如杀菌消炎、抗氧化、清热解毒等作用，有的已用于医疗和保健，因此，深入研究抱茎蓼挥发油有重要的价值和意义。本实验从秦巴山区野生抱茎蓼的花中提取挥发油，分析其组分并确定各个组分的相对含量，并运用正构烷烃系列物质对各组分