蓝玉簪龙胆对二甲基亚硝胺诱导的肝纤维化的治疗研究.docVIP

蓝玉簪龙胆对二甲基亚硝胺诱导的肝纤维化的治疗研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：蓝玉簪龙胆对二甲基亚硝胺诱导的肝纤维化的治疗研究 1 1 材料和方法 3 2 结果 5 3 讨论 6 文2：补肾柔肝方对二甲基亚硝胺诱导大鼠肝纤维化的预防作用及其机制研究 7 1 材料和方法 9 2 结 果 11 3 讨 论 11 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 蓝玉簪龙胆对二甲基亚硝胺诱导的肝纤维化的治疗研究 文1：蓝玉簪龙胆对二甲基亚硝胺诱导的肝纤维化的治疗研究 Abstract：ObjectiveTo investigate the protective effect of Gentiana veitchiorum on liver fibrosis induced by dimethylnitmsamine (DMN) in rats. Methods30 SD rats were randomly divided into control group，DMN group, Gentiana-treated group, prednisone-treated group and Gentiana+prednisone group．Rats in the DMN group, Gentiana-treated group, prednisone-treated group and Gentiana+prednisone group were successively administered intraperitoneally with DMN (10 mg/kg/d)，3 days per week for 4 weeks to induce hepatic fibrosis．At the same time，Gentiana-treated group was administered with Gentiana (4 g gruff/kg) ., prednisone-treated group was administered with prednisone (6 mg/kg) ., Gentiana+prednisone group was administered with Gentiana+prednisone (4g gruff+6mg/kg) . for 4 weeks respectively. Control group received normal sodium () only．The contents of ALT and HA in blood, SOD, MDA, GSH, MAO levels in hepatic tissue bomogenate were measured．Pathological changes with HE staining and MASSON staining were observed under light microscope．ResultsCompared with the model group, the contents of MAO, MDA declined significantly and the contents of GSH, SOD were increased significantly in Gentiana-treated group (P)．Fibrosis degree of liver was ameliorated when treated with Gentiana．ConclusionGentiana has anti-fibrosis effects agait liver injury induced by DMN and can be used in preventing the development of hepatic fibrosis by reducing the lipid peroxidation and suppressing the activation of hepatic satellite cells (HSC) Key words：Hepatic fibrosis； Gentiana veitchiorum； Dimethylnitmsamine 肝纤维化(hepatic fibrosis，HF)是继发于各种原因引起的肝脏炎症或损伤后组织修复过程中的代偿反应，以细胞外基质(extracellular matrix，ECM)在肝内过量