第四部分 从结果到成文.pptxVIP

医学生做基础科研，发表1-3 分文章的四个套路;课程内容;第四部分 高通量组学为主的研究;课程内容;一篇标准的芯片、测序文章;结果描述原则;整体——局部——个体——深入;50 papers;Overview of Gene expression profiles DEGs identification Hierarchical clustering/PCA analysis of DEGs Gene Function Annotation (GO and Pathway analysis) Network Construction (based on Interaction, Co-expression , Target Prediction. etc) Hub-Gene expression validation (with qPCR, WB, IHC , ELISA or GEO/TCGA database) Clinical Significance of Hub-gene as diagnostic or prognostic biomarker Novel Gene Functional Research in vivo or in vitro Up stream or down stream mechanism of Novel Gene in Disease;写