USP 621 CHROMATOGRAPHY 美国药典色谱.pdfVIP

Printed on: Wed Mar 16 2022, 01:57:02 AM(EST) Official Status: Not Yet Official on 16-Mar-2022 DocId: 1_GUID-6C3DF8B8-D12E-4253-A0E7-6855670CDB7B_2_en-US Printed by: ya huang Official Date: 01-Dec-2022 Document Type: GENERAL CHAPTER @2022 USPC DOI Ref: k9l64 DOI: /10.31003/USPNF_M99380_02_01 1 á621ñ CHROMATOGRAPHY Change to read: ▲INTRODUCTION Chromatographic separation techniques are multistage separation procedures in which the components of a sample are distributed between two phases, one of which is stationary while the other is mobile. The stationary phase may be a solid or a liquid supported on a solid or a gel. The stationary phase may be packed in a column, spread as a layer, or distributed as a film, etc. The mobile phase may be gaseous or liquid or supercritical fluid. The separation may be based on adsorption, mass distribution (partition), ion exchange, etc., or may be based on differences in the physicochemical properties of the molecules such as size, mass, volume, etc. l Portions of the present general chapter text that are national USP–NF text, and therefore not part of the harmonized text, are marked with symbols (♦♦) to specify this fact. ♦This chapter describes general procedures, definitions, and calculations of common parameters and generally applicable requirements for system suitability. a The types of chromatography useful in qualitative and quantitative analysis employed in USP procedures