生物化学与分子生物学实验15液质应用2013.pptxVIP

液相质谱技术(LC-MS)在生物学中的应用熊 英 2014年01月03日Multiple Dimension LC-MSThermo-Fishers, Linear Ion Trap MS (LTQ)linear ion Trap MS, LTQauto-samplercapillary LC separation system(located in Life Science Building)Bottom-Up Approachlinear proteinNCproteolysispeptide ions“Bottom-Up” ApproachThe most popular enzymes used in proteomicsNameCleaveDont cleaveN or C termTrypsinKRPCTERMChymotrypsinFYWPCTERMChymotrypsinFYWMLPCTERMPepsin (pH1.3)FL?CTERMPepsin (pH2)FLWYAEQ?CTERMLys-CKPCTERMLys-NKPNTERMGlu-CDEPCTERMAsp-ND?NTERMArg-CRPCTERMCNBrM?CTERMProteinase KAFYWLIVCTERMSeparation SystemSol A: 0.1 % Formic acid in