关于芪丹通脉片联合骨髓间充质干细胞移植对急性心肌缺血大鼠心肌细胞凋亡及心功能的影响.docVIP

关于芪丹通脉片联合骨髓间充质干细胞移植对急性心肌缺血大鼠心肌细胞凋亡及心功能的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：关于芪丹通脉片联合骨髓间充质干细胞移植对急性心肌缺血大鼠心肌细胞凋亡及心功能的影响 2 1 实验材料 3 2 实验方法 4 3 结果 6 2组大鼠心功能改变 6 2组大鼠移植后4周心肌细胞凋亡比较 7 2组大鼠移植后4周心肌缺血区微血管密度检测 7 4 讨论 7 文2：槐定碱对急性心肌缺血大鼠心功能的影响 7 1 材料与方法 9 2 结果 10 3 讨论 11 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 关于芪丹通脉片联合骨髓间充质干细胞移植对急性心肌缺血大鼠心肌细胞凋亡及心功能的影响 文1：关于芪丹通脉片联合骨髓间充质干细胞移植对急性心肌缺血大鼠心肌细胞凋亡及心功能的影响 Abstract：Objective To observe the effect of marrow mesenchymal stem cells (MSCs) traplantation combined with Qidantongmai tablet and simple MSCs traplantation on myocardial cells apoptosis and heart function in acute myocardial ischemia rats. Method Rat MSCs were isolated by deity gradient method， and then cultured and amplified to the third passage labeled with brdU. 16 SD rats were pided into two groups randomly， traplantation combined with Qidantongmai tablet group and simple traplantation group. The isochoric of suspeion of Qidantongmai tablet (2 g/kg) and were administered to SD rats by gastrogavage respectively for 14 days. The left anterior descending coronary artery of rat was ligated to establish animal models of acute myocardial infarction. The MSCs labeled with brdU were implanted intramyocardially into the infarct area. After 3 hou， the heart function were detected by an echocardiographic system (HP 5500 system)， equipped with a 12 MHz probe. After 4 weeks， the heart function were rechecked and MVD were detected by immunofluorescence， myocardial cells apoptosis were detected by TUNNEL. Results Heart function were improved in both groups， and was more significant in MSCs traplantation combined with Qidantongmai tablet group. Apoptotic index were decreased and the MVD of ischemic zone were increased in traplantation combined with Qidantongmai tablet group than simple traplantation group. Conclusion MSCs traplantation combined with Qidantongmai tablet could improve heart function of acute myocardial ischemia rats， decrease