分子生物学试卷(英文).pdfVIP

西南大学 生物技术学院 《Molecular Biology》课程试题 【A】卷 -0. 99 ～ 11/1/2021 学 年 第 1学 期 期末考试 考试时间 120 分钟 考核方式 开卷笔试 学生类别 本科 人数 98 适用专业或科类 生物技术 年级 05 级 题号 一 二 三 四 五 六 八 九 十 合计 得分 签名 阅卷须知：阅卷用红色墨水笔书写,得分用阿拉伯数字写在每小题题号前,用正分表示,不得分则在题号前写 0；大题得分 登录在对应的分数框内；统一命题的课程应集体阅卷 ,流水作业；阅卷后要进行复核,发现漏评、漏记或总分统计错误应 与时更正；对评定分数或统分记录进行修改时,修改人必须签名。 特别提醒：学生必须遵守课程考核纪律,违规者将受到严肃处理。 IDefine the meaning of these following terms 30 points, 3 pts per term 1. melting temperature Tm 2. C value paradox 3. exon 4. semi-conservation replication 5. cDNA library 6. gel shift assay 7. promoter 8. open reading frame ORF 9. gene family 10. expressed sequence tag EST II Fill in blank10 points, 1 pts per blank 1. The simplest cloning vector at least contains , and ori elements. 2. The diagram below shows two RNA molecules. Molecule I is processed to give molecule II. Answer the following questions about these two molecules: Where does this conversion happen? ____________________________ What type of RNA molecule is molecule I? _________________________ What type of RNA molecule is molecule II? _________________________ Name the regions/structures indicated by the arrows: A = _________________ B = _________________ C = _________________ D = _________________ E = _________________ III Select the right answer 20 points, 2 pts per question; Select th best answerfor each question. 1. The bond between a phosphate group and the ribose sugar groups in RNA is called which of the following? A Phosphodiester bondB Glycosidic bond C Peptide bond D Amide bond 2.Which enzyme does not play a role in DNA replication? A DNA polymerase B Helicase C Single-stranded binding protein D Guanyl transferase 3.