超滤柱说明课件.pptx

OMEGA超滤离心柱说明;Fractionation The molecules to be separated should differ by at least one order of magnitude (10X) in size for effective separation. ;How to Choose the Best Centrifugal Ultrafiltration Device;Choosing the Correct MWCO;Color-Coding;6;Nanosep and Nanosep MF Centrifugal Devices;Choosing the Appropriate Microsep? Device (Cont);使用说明;Diafiltration (Desalting and Buffer Exchange) For salt removal or buffer exchange, first concentrate sample at least 10-fold(i.e. 1 mL concentrated to 100 μL). Reconstitute with exchange buffer and reconcentrate 10-fold. Repeat this procedure 3 to 5 times to remove 95 to 99% of salt or buffer.;Sample Preparation for SDSElectrophoresis 1. Pipette 50 to 100 μL sample containing 5 to 60 μg of protein into Microsep device. 2. Dilute sample to 500ul with buffer. Spin to deadstop. Repeat twice. 3. Transfer concentrated sample to concentrate cup. Add SDS. Cap cup and heat to 80 °C for 10 minutes or more. 4. Remove from incubator or water bath and add ithiothreitol. Incubate at 56 °C. 5. Remove from incubator. Cool to room temperature. Prepare for layering on gel.;Chemical Compatibility