浅论硼替佐米抑制核因子κB活化对LOVO细胞增殖的影响（临床医学论文资料）.doc

浅论硼替佐米抑制核因子κB活化对LOVO细胞增殖的影响（临床医学论文资料） 文档信息 ： 文档作为关于“医学心理学”中“肿瘤学”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文12747字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：浅论硼替佐米抑制核因子κB活化对LOVO细胞增殖的影响 1 1材料和方法 3 文2：硼替佐米抑制核因子κB活化对LOVO细胞增殖的影响临床医学论文 9 1??材料和方法 10 2??结果 12 3??讨论 13 参考文摘引言： 16 原创性声明（模板） 17 文章致谢（模板） 17 正文 浅论硼替佐米抑制核因子κB活化对LOVO细胞增殖的影响（临床医学论文资料） 文1：浅论硼替佐米抑制核因子κB活化对LOVO细胞增殖的影响 Abstract: ? Objective: To investigate the influences of LOVO cells of the intervention of nuclear factor-κB (NF-κB) activation by bortezomib on the proliferation. Methods: LOVO cells was cultured in vitro and treated with different concentratio of bortezomib. The expressio of IκBα and NF-κB were analyzed by western blot and enzyme linked immunosorbent assay， respectively. The changes of cell growth and apoptosis were investigated by MTT assay and Annexin-V/PI double dyeing assay，respectively. Results: In bortezomib-treated cells，the expressio of IκBα and NF-κB in cytopalsm were obviously upregulated， while the level of NF-κB in nucleus was sharply downregulated in a dose-dependent manner (P?)， which was the most effective at the concentration of 80 nmol/L (P?). Bortezomib significantly inhibited the proliferation of LOVO cell in a dose-?and time-dependent manner. The percentage of apoptotic cells in bortezomib group was higher than that in control one (P?). Conclusion: Bortezomib can effectively intervene NF-κB activation in LOVO cells by suppressing the degradation of IκBα， inhibit the proliferation of LOVO cells and induce cell apoptosis。 Key words: ? bortezomib;colon carcinoma;nuclear factor-κB;LOVO;apoptosis 核因子-κB(nuclear factor-κB，NF-κB)是具有多向性调节作用的转录因子，参与细胞增殖与凋亡的调节，并且能直接影响细胞周期或DNA复制，从而参与体内多种生理和病理的过程。激活的NF-κB在炎症相关性结肠癌的发生发展中呈高表达，在慢性肠道炎症和癌变之间起桥梁作用[1]。硼替佐米(万柯，PS-341)是一种蛋白酶体抑制剂，主要用于治疗多发性骨髓瘤，其中的机制之一在于它能明显抑制NF-κB信号通路的活化。因此本研究以不同浓度的硼替佐米作用LOVO结肠癌细胞株，探讨硼替佐米对NF-κB活化的干预作用及对LOVO细胞增殖的影响。 1材料和方法 实验对象与主要试剂人LOVO结肠癌细胞株，由南京凯基生物科技发展有限公司提供。硼替佐米(Millennium，美国)购自西安杨森制药公司