Agilent Ultra-fast liquid chromatography using the Agilent 1100 Series HPLC system and 1.8-um ZORBAX SB C18 Rapid Resolution HT columns 说明书用户手册.PDF

Ultra-fast liquid chromatography using the Agilent 1100 Series HPLC system and 1.8-µm ZORBAX SB C18 Rapid Resolution HT columns Technical Overview Anabel Fandiño Stefan Schuette Abstract In recent years sample generation has rapidly increased both in chemical and pharmaceutical laboratories. Therefore, high-throughput analytical approaches are needed, not only in the discovery and development process but during the entire life cycle of a product. Purity, chemical and metabolic stability data as well as quality control data are primarily gained by means of liquid chromatography (LC). In order to get con- clusive LC analytical data swiftly, it is necessary to perform faster LC analyses in a reliable manner without losing chromatographic perfor- mance. In this publication we describe the recommended system con- figuration to achieve very fast analysis and cycle times, with and without alternating column regeneration by using ZORBAX 1.8-µm rapid resolu- tion high throughput (RRHT) columns on the Agilent 1100 Series liquid chromatograph. Performance and robustness of the system is exempli- fied by the analysis of a set of alkylphenones. We illustrate the baseline separation of 9 compounds within 29 seconds, achieving very sharp peaks of 0.5 seconds as well as outstanding resolution at backpressures of only 300 bar. Furthermore, performing a long-term study using alter- nating column regeneration with 8000 injections (4000 injections per column) we demonstrate the extremely long lifetime of our columns and reliability of the entire system. Introduction 6 µL. To further decrease cycle time needed for column flushing times, the overlapped injections 4