Agilent Enhanced Protein Identification with the Agilent Spectrum Mill MS Proteomics Workbench Version A.03.01 说明书用户手册.PDF

Enhanced Protein Identification with the Agilent Spectrum Mill MS Proteomics Workbench Version A.03.01 Technical Overview Jose Meza and John Chakel Agilent Technologies Introduction Mass spectrometry (MS) is widely used for protein with or without use of isotope labels. Also included identification. As MS techniques have become are homology mode searches, de novo sequencing, easier and more automated, the quantity of data and tools and utilities to speed proteomic generated has increased exponentially, necessitat- research. The Spectrum Mill workbench is an ing high-volume data processing tools such as the open platform that can process both Agilent and Agilent Spectrum Mill MS proteomics workbench. non-Agilent data. The Spectrum Mill workbench is a comprehensive set of high-throughput software tools for identify- The latest A.03.01 version of the Spectrum Mill ing proteins from MS and MS/MS spectra. This workbench extends the range of instruments for software can extract the high-quality peptide which the workbench can be used, and adds a spectra from raw data files, search the spectra number of new capabilities. This version of the against protein databases, and summarize the Spectrum Mill workbench is compatible with the results in ways that are meaningful to biologists Microsoft® Windows® Server 2003 and Windows and protein chemists. The summary capabilities 2000 Server operating systems. This technical allow ready comparison of multiple samples, each overview discusses the new features of Spectrum with multiple fractions, and