Agilent Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC TOF mass spectrometry 说明书用户手册.PDF

Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application Note Edgar Naegele Abstract A recent trend in the drug discovery and development process is to shift the starting point of drug metabolism and pharmacokinetic (DMPK) studies to a time as early as possible in the development chain to address potential issues in parallel with the optimization of the drug’s lead structure. There is a strong need for assay methods to determine early ADME parameters such as metabolic stability and metabolite identification. It is necessary to work with a fast LC/MS system which provides the necessary functionalities together with high speed and res- olution to handle the resulting large number of samples. In this Application Note a metabolic stability profile of buspirone will be acquired by the Agilent 1200 Series Rapid Resolution LC/TOF system on a short Rapid Resolution High Throughput (RRHT) column. Simultaneously, the main metabolites will be identified in the same experiment by the automated use of a long RRHT column, which provides the necessary high resolution. Introduction tem must be able to use multiple umn. Simultaneously, the main columns in a thermostatted col- metabolites will be identified in In modern pharmaceutical drug umn oven for various analytical the same experiment by the auto- development it is of crucial impor- methods and high throughput mated use of a long RR column, tance to know the adsorption, dis- functionalities such as overlapped which provides the necessary high tribution, metabolism and