embryoidbodiesfromembryonicstemcellsinvitro胚状体从胚胎干细胞体外培养.pptxVIP

Endoderm–Specific Gene Expression in Embryonic Stem–Cells Differentiated To Embryoid Bodies;Introduction;Past studies showed specific changes in expression of endoderm marker genes during EB development and suggested that EB formation could be considered an in-vitro model for endoderm differentiation.;Materials and Methods;LIF was withdrawn;Northern Blot Analysis;RT-PCR;Whole-mount in situ hybridization sectioning of EBs;Molecular Probes;Results;These data show that all the serum protein genes were activated at different stages, followed by a strong increase in expression, and all