甘草超高液相色谱指纹图谱研究.docxVIP

甘草超高液相色谱指纹图谱研究 摘要：目的建立甘草的超高液相色谱（uplc）指纹图谱，为其质量标准的建立提供依据。方法采用反相超高液相色谱法，以watersacquityuplcbehc18（1.7μm，2.1mm×100mm）为色谱柱，乙腈-0.05%磷酸为流动相，梯度洗脱，流速0.4ml/min，检测波长分别为237、355nm，柱温30℃，进样量2μl，记录时间51min。结果uplc指纹图谱的方法学考察结果符合技术要求。共标定甘草uplc指纹图谱的24个指纹峰，指认其中甘草酸、芹糖基异甘草苷、甘草查尔酮a、异甘草素、甘草苷、甘草素、异甘草苷、刺甘草查尔酮和光甘草定9个指纹峰。11批甘草样品指纹图谱在波长237nm处的相似度为0.940～0.988，波长355nm处的相似度为0.831～0.982。结论本研究建立的甘草uplc指纹图谱可为甘草质量标准的建立提供依据。 关键词：甘草;超高液相色谱;指纹图谱;甘草酸;相似度分析;质量标准 中图分类号：r284.1文献标识码：a文章编号：1005-5304（2020）01-0068-05 doi：10.3969/j.issn.1005-5304.201812371 studyonuplcfingerprintsofglycyrrhizaeradixetrhizoma sunyunbo1，2，zhangchuangfeng1，bidan1，tianqingcun3，cuixusheng3，shenshuo1，2 1.beijingyilingpharmaceuticalco.，ltd，beijing102600，china; 2.hebeiprovinceinstituteofintegratedtraditionalandwesternmedicine，shijiazhuang050035，china; 3.shijiazhuangyilingpharmaceuticalco.，ltd，shijiazhuang050035，china abstract：objectivetoestablishuplcfingerprintsofglycyrrhizaeradixetrhizoma，andtoprovideabasistodevelopthequalitystandard.methodsrp-uplcfingerprintswereadopted，andwatersacquityuplcbehc18（1.7?m，2.1mm×100mm）wassetasthechromatographiccolumn.acetonitrile-0.05%phosphoricacidwasusedasthemobilephase，forgradientelution;theflowratewas0.4ml/min;thedetectionwavelengthsweresetat237nmand355nm;thecolumntemperaturewaskeptat30℃;thesamplevolumewas2μl;therecordingtimewas51min.resultsthemethodologicalfindingsofuplcfingerprintsmetthetechnicalstandard.24fingerprintpeaksweremarkedinglycyrrhizaeradixetrhizoma.9fingerprintpeaksincludingglycyrrhizicacid，isoliquiritinapioside，licochalconea，isoliquiritigenin，liquiritin，liquiritigenin，isoliquiritin，echinatinandglabridinwereidentified.thesimilaritiesof11batchesofglycyrrhizaeradixetrhizomawereamong0.940–0.988at237nmandamong0.831–0.982at355nm.conclusiontheestablisheduplcfingerprintsofglycyrrhizaeradixetrhizomacanprovidebasisfortheestablishmentofitsqualitystandard. keywords：glycyrrhizaeradixetrhizoma;uplc;fingerprints;glycyrrhizicacid;similarityanalysis;qualitystanda