诺唯赞试剂说明书 One Step Cloning kit.docxVIP

PAGE 7 ClonExpress? II One Step Cloning kit Catalog # C112 1. Introduction ClonExpress? One Step Cloning Technology ClonExpress? One Step Cloning Technology is a simple, fast and highly efficient cloning kit which is based on homologous recombination technology. It allows to directly clone any amplified product(s) to any linearized vector, at any site. Firstly, the expression vector will be linearized at the cloning site of choice. A small sequence (15-20 bps) overlapped with the end of the cloning site will be added onto the insert through a PCR step. After the inserts and the linearized vector are mixed in the presence of Exnase? for only 30 minutes, the cloning DNA products can be directly subjected to E.coli transformation with true positive rate over 95%. ClonExpress? II One Step Cloning kit is a new version cloning kit based on ClonExpress? One Step Cloning technology，supplied with Exnase? II and reaction buffer. Compared to the previous version, Exnase? II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even using low efficient competent cells (107 cfu/μg). Additionally, Exnase? II is also compatible with the endonuclease digesting reaction and PCR reaction assay. Digested products or PCR products can be directly applied in recombination reaction without purification, which significantly simplifies the experimental procedures. Product advantages -Easy and fast -Highly efficiency. -Applicable to multiple insert assembly at any cloning site of choice without restriction endonuclease -Sequentially assemble up to five inserts in a single reaction Application -Fast assembly -High-through assembly -Seamless assembly -Site-specific mutagenesis 2. Package Information Components C112-01 (25 rxn) C112-02 (50 rxn) 5 × CE II Buffer 100 μl 200 μl Exnase? II 50 μl 100 μl 500 bp control insert(25ng/μl) 5 μl 5 μl pUC19 control vector, linearized (50 ng/μl) 5 μl 5 μl 3. Storage Store at -20°C