川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响.docVIP

川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响 目录 TOC \o "1-9" \h \z \u 目录 1 正文 2 文1：川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响 2 1 材料 3 1.1 实验动物及分组 3 1.2 仪器与材料 4 2 方法 4 文2：川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响及其治疗作用 5 1 材料 7 1.1 实验动物及分组 7 1.2 仪器与材料 7 2 方法 8 2.2 NO测定 8 2.3 肺组织iNOS活性的测定 8 2.4 组织学检查 9 2.5 统计学处理 9 3 结果 9 3.1 肺湿/干重比、中性粒细胞比、蛋白含量测定 9 3.2 肺组织NO和iNOS的变化研究 9 3.3 组织病 理学 改变 10 4 讨论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响 文1：川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响 Abstract：ObjectiveThe variance of NO and iNOS in Paraquat-induced acute lung injured rats was investigated, and the protection of ligustrazine on rat acute lung injury was approached.Methods50 Sprague- Dawley (SD) rats were randomly divided into five experimental groups: blank group, negative control group, positive control group, ligustrazine low-dose group(LDG), ligustrazine high-dose group(HDG). Macroscopic and histopathological examinatio were performed and biological indexes were measured for the lung specime. The indexes included lung wet weight/dry weight, the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the contents of lung tissue homogenate NO and iNOS were measured. ResultsCompared with negative control group, lung congestion and lung edema of ligustrazine low-dose group were mitigated. Lung wet weight/dry weight, neutrophil infiltration, and protein content were decreased (P＜0.05 or P＜0.01), and biological indexes of lung injury and the contents of NO and iNOS were significantly decreased (P＜0.05 or P＜0.01). ConclusionNO and iNOS play important roles in lung injury of poisoned rats. Ligustrazine can ameliorate the lung injury and decrease the concentratio of NO and iNOS. Key words：Ligustrazine; Paraquat; Pulmonary injury; Nitric oxide(NO); Inducible nitric oxide synthase (iNOS) 百草枯（又名Paraquat, PQ，克无踪，克灭踪等）是有机杂环类接触性脱叶剂及除草剂，随着其广泛使用，引起大量中毒事件的发生，死亡率可高达85%～95%［1］ 。百草枯在肺组织中浓度比血浆浓度高10～9