PLK1在急性白血病细胞中的表达及意义.docVIP

PLK1在急性白血病细胞中的表达及意义 正文 PLK1在急性白血病细胞中的表达及意义 文1：PLK1在急性白血病细胞中的表达及意义 Expression of plk-1 Gene in Acute Leukemia Patients and Its Significanc AbstractTo investigate expression of plk-1 gene and PLK-1 protein in acute leukemia patients and its clinical significance, and to observe the distribution of PLk-1 protein in acute leukemin cells, the mononuclear cells were separated from the bone marrow or peripheral blood of acute leukemia patients, bone marrow benign proliferation individuals and normal individuals. The expression of plk-1 gene and PLK-1 protein in those cells were detected with RT-PCR and flow cytometry respectively, the distribution pattern of PLK-1 was observed by fluorescent inverted microscope. The result showed that the expressio of plk-1 gene and PLK-1 protein in mononuclear cells of acute leukemia patients were much higher than that of bone marrow benign proliferation individuals and normal individuals. Fluorescent inverted microscopy revealed that PLK-1 was highly concentrated in cytoplasm of acute leukemia cells during interphase of mitosis, and it was found that PLK-1 was mainly distributed between sister chromatid during the mitosis in mononuclear cells of acute leukemia patients, but the expressio of plk-1 gene and PLK-1 protein almost were not observed in cells of benign proliferative bone marrow and normal bone marrow. It is concluded that increased plk-1 gene and protein perhaps play an important role in abnormal proliferation of acute leukemia cells and correlate with the malignamcy of leukemia. plk-1 gene or PLK-1 protein may be coidered as a new target of therapy, and one of useful indicato in evaluation of curative effeciency and prognosis. Key wordacute leukemia; Plk-1 gene; PLK-1 protein; acute leukemia cell; gene expression 肿瘤的发生、发展与细胞周期的紊乱有关，polo-样激酶-1（polo-like kinase-1, PLK-1）是一种高度保守的丝氨酸/苏氨酸激酶,主要参与染色体纺锤体的形成及分配,它对细胞周期的正常进行、中心体功能成熟、胞质分裂起监控调节作用［1,2］。PLK-1在实体瘤细胞中呈现高表达并且对肿瘤的发生、发展起重要作用［ 3］，而在急性白血病发病中的表达情况鲜有报告。为此我们进行了实验，旨在研究plk-1基因在急性白血病细胞中的