前列腺导管腺癌35例基底细胞变化的形态学.docVIP

前列腺导管腺癌35例基底细胞变化的形态学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：前列腺导管腺癌35例基底细胞变化的形态学 1 0 引言 2 1 材料和方法 3 2 结果 4 2.2 免疫组化检测结果 4 文2：前列腺导管腺癌35例基底细胞变化的形态学 5 0 引言 6 1 材料和方法 6 2 结果 7 2.2 免疫组化检测结果 8 3 讨论 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 前列腺导管腺癌35例基底细胞变化的形态学 文1：前列腺导管腺癌35例基底细胞变化的形态学 【Abstract】 AIM: To investigate the clinicopathological features of basal cells in prostatic duct adenocarcinoma which can help pathological diagonosis of prostatic cancer. METHODS: Thirtyfive cases of prostatic duct adenocarcinoma were studied by hematoxylin and eosin staining and immunohistochemical techniques of twostep ElivisionTM Plus method to detect the expression of 34βE12, P63, P504S, PSA and PSAP. And the abscence of basal cells in prostatic duct adenocarcinoma was analysed according to the immunohistochemical staining. RESULTS: Of the total 35 cases, nearly intact basal cells were observed by immunohistochemical staining in 13 cases;only small amounts of incomplete basal cells in 3 cases; and complete absence of base cells in 19 cases 27 cases with P504S positive, 34βE12 and P63 negative staining at the edge of small invasive carcinoma and/or small acinous carcinoma around the prostatic duct adenocarcinoma were also observed. CONCLUSION: Although obvious absence of basal cells is usually observed in prostate duct adenocarcinoma, some basal cells still remain in the tumor tissues, which shows that the absence of basal cells is not the most significant index on the pathological diagnosis of prostate duct adenocarcinoma, and too much dependence on the importance of basal cell absence in diagnosis of prostate duct adenocarcinoma will lead to misdiagnosis and missed diagnosis. 【Keywords】 prostatic neoplasms; duct adenocarcinoma; basal cell; immunohistochemistry 0 引言 前列腺导管腺癌（prostate duct adenocarcinoma, PDA）为前列腺腺癌的一种亚型. 随着前列腺癌穿刺活检的广泛开展, 前列腺癌诊断的复杂性日益受到病理工作者的重视. 本实验通过观察35例PDA HE切片的镜下结构和基底细胞免疫组化的表达特点，探讨该类前列腺癌中基底细胞的缺失比率、免疫组化表达特点、肿瘤生长方式及导管腺癌周围混合癌成