复方参七汤对胃结直肠癌抑制作用的实验研究(临床医学范文).docVIP

复方参七汤对胃结直肠癌抑制作用的实验研究(临床医学范文).doc

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复方参七汤对胃结直肠癌抑制作用的实验研究(临床医学范文) 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1:复方参七汤对胃结直肠癌抑制作用的实验研究 1 1 材料与方法 3 1.2 方法 3 2: 5-GTTTTCTGCGCAAGGTTAGG-3 6 文2:复方参七汤对胃结直肠癌抑制作用的实验研究临床医学论文 6 1 材料与方法 8 1.2 方法 8 2: 5-GTTTTCTGCGCAAGGTTAGG-3 11 2 结 果 11 3 讨 论 13 参考文摘引言: 14 原创性声明(模板) 16 文章致谢(模板) 16 正文 复方参七汤对胃结直肠癌抑制作用的实验研究(临床医学范文) 文1:复方参七汤对胃结直肠癌抑制作用的实验研究 [Abstract] Objective: To investigate the mechanism of therapeutic effect of compound Shenqitang on the gastric and colorectal carcinomas. Methods: Several experiments had been done, including:(1) the inhibitory effect of compound Shenqitang on human gastric adenocarcinoma cell line in vitro;(2) its inhibitory effects on Wistar rat gastric adenocarcinoma induced by MNNG; and (3) its inhibitory effect on the hepatic metastases of colorectal carcinoma in mice. Results: (1)Obvious inhibitory effects of compound Shenqitang had been found on the cell division and colony form of human gastric adenocarcinoma cell line BGC-823 in vitro. The LC50 was 0.007g crude drug/ml in colony form experiment. The drug had potent killing effect in higher concentration, with LC50 of 0.048g crude drug/ml;(2)The incidence of gastric adenocarcinoma in the experimental group(6.9%) was significantly lower than that in the control group(58.9%).The positive rates of PCNA and cyclin D1 in the experimental group were 13.88%(5/36) and 22.86%(8/35), which were significantly lower than those in the control group(P0.01);(3)Compound Shenqitang inhibited hepatic metastases of colorectal carcinoma in mouse model. Nm23H1-NDPK positive expression rate (66.67%) was significantly higher in the experimental group than that (16.67%) in the control group(P0.05). Conclusion: Compound Shenqitang can effectively kill the human gastric adnocarcinoma cell line BGC-823 in vitro, inhibit the incidence of the gastric adenocarcinoma induced by MNNG in Wistar rats, and inhibit hepatic metastasis of colon carcinoma in mouse model. [Key words] Gastric carcin

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