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Materials and methods Cell culture and antibody HL-60 leukemic cells were grown in suspension in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 0.5% antibiotics and 2.0 g/L sodium bicarbonate in a 5% CO2 humidified incubator at 37 °C. The monoclonal antibody (MAb) to nuc