猪戊型肝炎病毒SYBR Green Ⅱ荧光定量RTPCR检测方法的建立与应用.pdfVIP

猪戊型肝炎病毒 SYBR Green Ⅱ荧光定量 RT-PCR 检测方法 的建立与应用 李幽幽;龚双燕;李小璟;毛汐语;邓益超;朱玲;徐志文 【摘要】In order to establish a real-time RT-PCR based on SYBR Green Ⅱ for detection of hepatitis E virus (HEV),a pair of special primers was designed according to the conserved sequences of ORF2 in GenBank.Result showed that the standard curve of established SYBR Green Ⅱ real-time RT-PCR had a wide dynamic range from 4.10 × 102-4.10 × 108 copies/μL with a linear correlation(r2) of 0.996.The sensitivity could reach 1.00 × 102 copies/μL.The melting curve analysis using SYBR Green Ⅱ dye