昆虫材料扫描电镜观察技术.docxVIP

昆虫材料扫描电镜观察技术宋月芹;董钧锋;孙会忠【摘要】Themaintechnicalaspectsandnotesoftheobservationoninsectmaterialwithscanningelectronmicroscope(SEM)wereintroduced.ThemainprocessofobservationbySEMincludespretreatmentofthematerial,doublefixation,clean,dehydration,replacement,drying,gluestation,sprayingmetalcoatingandobservation.Throughthecontrastofexperimentalpictures,thekeytechnologyforobtaininggoodobservationeffectwassummarizedanddiscussed.Theobservationtechnologyhaswidepopularizationvalueandreferencesignificance.%介绍了昆虫材料扫描电子显微镜观察的主要技术环节及注意事项.扫描电镜观察主要包括材料的预处理、清洗、二重固定、脱水、置换、干燥、粘台、喷金镀膜和观察等步骤.并通过与实例图片的对比，总结和讨论了获得较好观察效果