蛋白酶的工厂设计.pdfVIP

年产 1500m³蛋白酶的工厂设计 摘 要 蛋白酶是催化肽键水解的一类酶，它可迅速水解蛋白质为胨、肽类，广 泛存在于动物内脏、植物茎叶、果实和微生物中。同时大多数微生物蛋白酶 都是胞外酶。微生物蛋白酶按其作用的最适 pH 可分为酸性蛋白酶、中性蛋 白酶、碱性蛋白酶三类。酸性蛋白酶是一种羧基蛋白酶，它的分子质量为 30-40kD，等电点（pH3.0-5.0） 酸性蛋白酶现已广泛应用于食品、饲料、酿造、毛皮与皮革、医药、胶 原纤维等各个行业之中。本设计采用豆饼粉、玉米粉、淀粉为主要的培养基 原料，并选用黑曲霉（Aspergillus niger ）3.350 菌种发酵。其中豆饼粉 3.75%，玉米粉 0.625%，鱼粉 0.625%，氯化铵 1%，氯化钙 0.5%，磷酸氢二 钠 0.2%。 本设计利用通风搅拌式发酵罐进行发酵，同时利用离子交换树脂对母液 进行提取，提高了酸性蛋白酶的生产效率，减少了生产成本。设计还包括发 酵罐，全厂平面图，车间平面布置图，工艺流程图。 关键词：酸性蛋白酶 发酵 工厂设计 1 The Process Design of the Protease used for Section with the Capacity of 1500m³ Annually Abstrac protease is a kind of Peptone and peptide. It has been discover across in animal giblets ,the stem of plant,fruit , microbial and so on.Most of the Microbial protease are ectoenzyme .According to its best Optimum pH function ,Microbial protease Can be divided into Acid protease ,Neutral protease and alkaline protease .Acid protease is a kind of Carboxyl protease , Its molecular weight is 30-40 kd, lower isoelectric point (pH3.0-5.0) Acid protease in food, medicine, textile, leather, feed, cosmetics, washing industries have applications, natural health, avirulent and harmless, quite safe. So in this paper the basic content of more acid protease, production process and application development were introduced. This design USES the bean cake powder, corn flour, starch as the main medium of raw materials, and selects the Aspergillus Niger, Aspergillus Niger) 3.350 bacterial fermentation. With bean cake powder 3.75%, corn flour 3.75%, 0.625% fish meal, 1% ammonium chloride, calcium chloride 0.5%, disodium hydrogen phosphate 0.2%. This design using the ventilation agitato