乙酰肝素酶反义寡核苷酸对肺癌a.pdfVIP

乙酰肝素酶反义寡核苷酸对肺癌A 549 细胞侵袭力 的抑制作用 作者：陈志涛 田 辉1 岳韦名1 李 林1 亓 磊1 朱应超1 作 者单位：(山东大学附属济南市中心医院胸外科，山东 济南 250013) 【摘要】目的探讨乙酰肝素酶(heparanase，HPSE )反义寡 核苷酸(antisense oligodeoxynucleotide，ASODN)对人肺癌A 549 细 胞侵袭力的抑制作用。方法 设计合成互补于HPSE mRNA 起始密码 区的HPSE ASODN ，以阳离子脂质体Lipofectin 包埋后转染人肺癌A 549 应(RT PCR) 方法检测肺癌A 549 细胞HPSE 蛋白及其mRNA 表 达的变化;以Matrigel 细胞侵袭试验对HPSE AS ODN 对肺癌A 549 细胞侵袭的抑制进行检测。结果 ASODN 各组与正常对照组和脂 质体组比较及ASODN 各组间比较HPSE mRNA 和蛋白的表达及细胞 侵袭力均受到明显抑制 (P0.01);ASODN 在终浓度为 100、200 、400 nmol/L 时对肺癌A 549 细胞侵袭力的抑制率分别为 55.6%、82.3% 和91.2% 。结论 HPSE ASODN 通过下调 HPSE mRNA 和蛋白的表达 可显著抑制肺癌A 549 细胞的侵袭力，并呈剂量依赖性。 【关键词】 肺肿瘤;乙酰肝素酶; 反义寡核苷酸 Inhibition of invasiveness of human lung cancer cell lines by heparanase antisense oligodeoxynucleotide CHEN Zhi Tao ，TIAN Hui ，YUE Wei Ming ，et al. Department of Thoracic Surgery ，Ji ′nan Central Hospital Affiliated to Shandong University ，Ji ′nan 250013 ，Shandong，China 【Abstract 】Objective To evaluate the inhibitory effect of HPSE antisense oligodeoxynucleotide (ASODN) on the invasiveness of human lung cancer A 549 cell lines.Methods HPSE ASODN which was complementary with initiation codon region of HPSE mRNA was designed and synthesized.After embedded by cation lipofectin ，it was transfected into A 549 cells of human lung cancer.The expression of HPSE protein and HPSE mRNA in A 549 cell lines were detected by flow cytometry and RT PCR.Meanwhile Matrigel invasive assay was used to measure the inhibitory effect of HPSE ASODN on the invasiveness of human A 549 cell lines.Results The HPSE protein and HPSE mRNA expression and invasiveness of human A 549 cells treated with ASODN of different concentrations were significantly decr