海洋普鲁兰短梗霉HN6.2菌株铁载体合成与调控的研究.docxVIP

海洋普鲁兰短梗霉HN6.2菌株铁载体合成与调控的研究 摘要：铁是细胞生长和代谢所必需的微量元素。海洋普鲁兰短梗霉HN6.2菌株是一种生长在海洋环境中，具有生物活性化合物合成能力的真菌。 本研究中，我们分析了海洋普鲁兰短梗霉HN6.2菌株对铁的需求，以及其如何适应低铁环境和合成铁载体的能力。我们建立了一个含有不同铁浓度的培养基，并使用不同生物学技术，如RT-qPCR和全转录组测序，来研究海洋普鲁兰短梗霉HN6.2菌株在不同的铁供应条件下的铁代谢调控机制和铁载体的合成。研究结果表明，海洋普鲁兰短梗霉HN6.2菌株中的铁代谢受到羟基基酸合成、硫代硫酸盐合成和溶菌酶降解等多个代谢通路的影响，并发现了一些新的铁代谢相关基因和通路。我们还探索了海洋普鲁兰短梗霉HN6.2菌株的铁载体合成和调控机制，并发现了多个铁载体基因及其调控网络。这些研究成果可能为理解铁代谢和铁载体合成在海洋环境中的生物学意义提供新的思路。 关键词：铁代谢；铁载体；海洋普鲁兰短梗霉HN6.2菌株；溶菌酶 Abstract: Iron is a trace element that is essential for cell growth and metabolism. Marine Penicillium brevicompactum HN6.2 is a fungus that grows in the marine environment and produces bioactive compounds. In this study, we analyzed the iron requirement of HN6.2 and its ability to adapt to low iron environment and synthesize iron carriers. We established a culture medium with different iron concentrations and used different biological technologies, such as RT-qPCR and transcriptome sequencing, to study the iron metabolism regulation and iron carrier synthesis of HN6.2 under different iron supply conditions. The results showed that iron metabolism in HN6.2 was influenced by multiple metabolic pathways such as hydroxyl acid synthesis, sulfate synthesis, and lysozyme degradation, and new iron metabolism-related genes and pathways were discovered. We also explored the synthesis and regulation mechanism of iron carriers in HN6.2 and found multiple iron carrier genes and their regulation networks. These results may provide new insights into the biological significance of iron metabolism and iron carrier synthesis in the marine environment. Keywords: iron metabolism; iron carrier; marine Penicillium brevicompactum HN6.2; lysozyme