DB37_T 3570-2019奶牛A2型β-酪蛋白基因检测技术规程.pdf

ICS65. 020. 01B 40DB37山東东省地方标准DB 37/T 3570—2019奶牛A2型β-酪蛋白基因检测技术规程Code of practice for detection of A2 type of β-casein gene in dairy cattle2019-05-29发布2019-06-29实施山东省市场监督管理局发布 DB37/T次前言1I1范围.2规范性引用文件3术语和定义4检测原理5主要试剂，6主要仪器设备7检测方法7.1样本采集7.2 DNA提取.7.3多重PCR扩增7.4多重连接酶检测反应7.5变体型分析8结果判定8.1多重PCR扩增产物的检测8.2A2型β-酪蛋白结果判定9废弃物的处理10检测过程中防止交叉污染的措施附录A（资料性附录）主要试剂配制附B（资料性附录）主要仪器设备附C（规范性附录）多重连接酶检测反应探针引物 DB37/T 3570—2019附录C（规范性附录）多重连接酶检测反应探针引物引物序列(5° -3 )扩增长度(bp)检测SNP位点TTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATTGAGAAG1TTCAGAGTGL6TTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATTGAGAAGTTTCAGAGTA99c, 151 GAPAGGAACAGCAGCAAACAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTFAMTTTTTTTTTTTTTTTTATTACCTCTGTTTGCTGCTGTIT77TTTTTTTTTTTTTTTTTTATTACCTCTGTTTGCTGCTGTTC62c. 154 GAPCTCACTCTGAAACTTCTCAATTTTTTTTTTTTTTTTTFAMTTTTTTTTTTTTTTTTTTTTGATGTTTTGTGGGAGGCTGTTAT105TTTTTTTTTTTTTTTTTTTTTTTTTTTTGATGTITTGTGGGAGGCTGTTAG107c. 245 CAPGGATGGGTCCAGGGAAGGGATTTTTTTTTTTTTTTTTTTTTITITFAMTTTTTTTTTTTTTTTTTTTACTCCCATTACTTCAGGCTGAAT85TTTTTTTTTTTTTTTTTTACTCCCATTACTTCAGGCTGAAG87c, 307 CAPGAAAGGCGGCACCACCACAGTTTTTTTTTTTTTTTTTTTTTTFAMTTTTTTCCTTCACTTTGGAGACTCCCAT81TTTTTTTTTTTCCTTCACTTGGAGACTCCCAG83c. 322 ACPTACTTCAGGCTGAAGGAAAGTTTTTTTTTTTTTTTTTTTFAMTTTTTTTTTAGGAGGCTATGGCTCCTAAGCAC89TTTTTTTTTTTTTTTTTTTTTTTTAGGAGGCTATGGCTCCTAAGCAA91c. 363 cAPAAAGAAATGCCCTTCCCTAATTTTTTTTTTTTTTTTTTTTTFAMTTTTTTTTTTTTTTTTTTTCATCAGTGAGAGTCAGGCTCTGG93TTTTTTTTTTTTTTTTTTTTTTCATCAGTGAGAGTCAGGCTCTGC95c. 411 cGPCTTTCAGTAAAGGGCTCAACTTTTTTTTTTTTTTTTTTTTTTTTTTFAMTTTTTTTAGGAGGAAACATGACAGTTGGAA1OTTTTTTTTTTTTTTAGGAGGAAACATGACAGTTGGAG103c, 500 CTPGAAGAGGCTGGTGAGGCTGGTTTTTTTTTTTTTTTTTTTTTTTTTITTFAM DB37/T言本标准按照GB/T1.1—2009给出的规则起草，本标准由山东省畜牧兽医局提出并监督实施。本标准由山东省畜牧业标准化技术委员会归口。本标准起草单位：山东省农业科学院奶牛研究中心、山东省备牧总站、山东奥克斯备牧种业有限公司、大同市南郊区四方高科农牧有限公司、中国农业大学、江苏省农业科学院备牧研究所。本标准主要起草人：黄金明、王秀革、翰志花、要强、王玲玲、张思聪、张亚再、高亚平、魏晓超刘文浩、高运东、侯明海、曲绪仙、段志伟、戴蕴平、仲峰。1I DB37/T 3570—2019奶牛A2型β-酪蛋白基因检测技术规程1范围本标准规定了奶牛A2型β-酪蛋白基因检测的技术方法。本标准适用于奶牛A2型β-酪蛋白基因的检测与分型。规范性引用文件下列文件对于本文件的应用是必不可少的。凡是注日期的引用文件，仅所注日期的版本适用于本文件。凡是不注日期的引用文件，其最新版本（包