马立克肿瘤及接种火鸡疱疹病毒活疫苗后的SPF鸡微卫星不稳定性的研究的开题报告.docxVIP

马立克肿瘤及接种火鸡疱疹病毒活疫苗后的SPF鸡微卫星不稳定性的研究的开题报告 【摘要】 鸡肿瘤是鸡养殖业中常见的疾病之一，而疱疹病毒活疫苗是常用的预防措施之一。本研究旨在探究接种火鸡疱疹病毒活疫苗后，马立克肿瘤SPF鸡的微卫星不稳定性的变化情况。通过PCR和DNA测序技术，分析SPF鸡在疫苗接种前后微卫星位点的变异情况，并对不稳定性的原因进行探讨。结果有望为深入研究鸡肿瘤发病机制和疫苗防治策略提供理论依据。 【关键词】马立克肿瘤；接种；火鸡疱疹病毒活疫苗；微卫星不稳定性；PCR；DNA测序 【Abstract】 Chicken tumor is one of the common diseases in chicken farming, and herpesvirus live vaccine is one of the commonly used preventive measures. The purpose of this study is to explore the changes in microsatellite instability of SPF chickens with Mareks disease tumor after vaccination with turkey herpesvirus live vaccine. By using PCR and DNA sequencing technology, the variation of microsatellite loci before and after vaccination in SPF chickens was analyzed, and the reasons for instability were discussed. The results are expected to provide a theoretical basis for in-depth study of the pathogenesis of chicken tumors and vaccine prevention strategies. 【Key words】 Mareks disease tumor; vaccination; turkey herpesvirus live vaccine; microsatellite instability; PCR; DNA sequencing