hIL-10基因修饰骨髓间充质干细胞对大鼠异种肝移植的影响的开题报告.docxVIP

hIL-10基因修饰骨髓间充质干细胞对大鼠异种肝移植的影响的开题报告 摘要： 本文旨在探讨骨髓间充质干细胞（BMSCs）通过IL-10基因修饰对大鼠异种肝移植的影响。首先，采集大鼠BMSCs，通过质粒转染技术将IL-10基因转染进入BMSCs中，然后进行细胞鉴定。随后，将大鼠分为3组，分别是BMSCs-IL-10组、BMSCs组和PBS组。将异种肝移植进入大鼠，并在手术后分别注射BMSCs-IL-10、BMSCs或PBS，跟踪观察大鼠移植肝功能的变化、细胞因子的分泌和组织病理变化。 研究结果显示，BMSCs-IL-10组的大鼠移植肝功能恢复更快，血清转氨酶显著降低，而组织病理学检查也显示其中的炎症水平更低。同时，BMSCs-IL-10组的大鼠体内IL-10、IL-6、TNF-α和IFN-γ等因子的分泌显著低于其他两组。 因此，本研究认为，BMSCs通过IL-10基因转染后，可以显著降低大鼠异种肝移植的炎症反应，促进移植肝的修复和功能恢复。 关键词：骨髓间充质干细胞；IL-10基因；大鼠异种肝移植；炎症反应；移植肝的修复和功能恢复。 Abstract： The present study aims to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) modified with IL-10 gene on allograft liver transplantation in rats. First, rat BMSCs were collected and transfected with IL-10 gene by plasmid transfection technology, and then cell identification was performed. Then, rats were divided into three groups, BMSCs-IL-10 group, BMSCs group and PBS group. The allograft liver was transplanted into the rats, and BMSCs-IL-10, BMSCs or PBS were injected after surgery. Changes in graft liver function, cytokine secretion and histopathological changes were tracked and observed. The results showed that the rats in the BMSCs-IL-10 group had faster recovery of graft liver function, significant decrease in serum transaminases, and lower inflammation levels in histopathology examination. At the same time, the secretion of IL-10, IL-6, TNF-α and IFN-γ in the body of rats in the BMSCs-IL-10 group was significantly lower than in the other two groups. Therefore, this study believes that BMSCs modified with IL-10 gene can significantly reduce the inflammatory reaction of allograft liver transplantation in rats, promote the repair and function recovery of graft liver. Keywords: bone marrow mesenchymal stem cells; IL-10 gene; allograft liver transplantation in rats; inflammatory response; repair and function recovery of graft liver.