肠炎沙门氏菌热损伤修复方法研究及其rpoH基因的敲除的开题报告.docxVIP

肠炎沙门氏菌热损伤修复方法研究及其rpoH基因的敲除的开题报告 摘要： 肠炎沙门氏菌属于沙门氏菌科，是一种常见的肠道病原菌，能够引起人类和动物的肠道感染。肠炎沙门氏菌热损伤是该菌感染的主要病变之一，其热损伤机制和修复机制目前还不够清楚。本研究旨在探究肠炎沙门氏菌热损伤的修复机制，通过敲除rpoH基因，研究其在热适应中的作用。 本研究将采用肠炎沙门氏菌的基因敲除技术，构建rpoH基因敲除菌株，并对其进行热适应实验，观察其热适应能力的变化。同时，通过各种技术手段对敲除菌株中相关修复机制的基因表达、蛋白质表达进行分析，探究敲除rpoH基因对菌体内热适应修复机制的影响。 预期研究结果是：敲除rpoH基因会使得肠炎沙门氏菌失去热适应的能力，进而影响菌体内相关的热适应修复机制。同时，通过进一步的基因表达、蛋白质表达分析，可以探究rpoH基因在菌体内的作用机制以及相关热适应修复机制的基因网络。 关键词：肠炎沙门氏菌；热损伤修复；rpoH基因；敲除技术；热适应能力；基因表达；蛋白质表达 Abstract: Salmonella enteritis is a commonly seen intestinal pathogenic bacteria, which can result in intestinal infection in humans and animals alike. Salmonella enteritis heat injury is one of the major lesions caused by this bacterium, however, its injury and repair mechanisms remain poorly understood. The current study aims to investigate the repair mechanisms involved in Salmonella enteritis heat injury, and evaluate the role of rpoH gene in thermal adaptation using gene knockout technique. Gene knockout methodology will be utilized to create a rpoH knockout strain for Salmonella enteritis. Thermal adaptation experiments will then be carried out to assess its ability compared to the wildtype strain. Following this, analysis of the gene and protein expression associated with functional repair mechanism will be carried out in the rpoH knockout strain to analyze the effects of disabling the expression of the rpoH gene. The study is expected to demonstrate the loss of thermal adaptation in the rpoH knockout strain, as well as its effects on related repair mechanism within the bacterium. Analysis of the gene and protein expression will also enable us to study the functional mechanism of rpoH gene in the bacterium and the gene network of thermal adaptation repair mechanism. Key words: Salmonella enteritis; thermal injury repair; rpoH gene; gene knockout technique; thermal adaptation ability; gene expression; protein expression.