毛白杨抗冻性低温诱导过程中糖的效应_英文_.docVIP

毛白杨抗冻性低温诱导过程中糖的效应_英文_ Effect of Sugar on the Process of Cold2Acclimation2Induced Freezing Tolerance of Popul us tome ntos a 3Zhang Zhiyi L in Shanzhi Populus tomentosa seedlings for cold2acclimating were pretreated with or without 20 % saccharose . ABSTRACT Changes in the concentrations of total soluble sugar , the survival rates , and freezing tolerance of seedlings during cold acclimation were investigated. The results showed that cold acclimation increased the concentrations of total soluble sugar , the survival rates and freezing tolerance . Cold acclimation , combined with the saccharose2pretreat2 ment , enhanced the above2mentioned effect of cold acclimation , and obviously increased the concentrations of total soluble sugar , the survival rates and freezing tolerance of seedlings. Further analysis found that the concentrations of total soluble sugar in branches increased greater than that in leaves during both cold acclimation with or without the pretreatment of saccharose . Moreover , an increase of the concentrations of total soluble sugar in branches and leaves was closely related to the freezing tolerance of seedlings. The results indicate the accumulation of soluble sugar in seedlings induced by cold acclimation may be involved in the induction of freezing tolerance . KEY WO RDS Populus tomentosa , cold acclimation , freezing tolerance , survival rates , soluble sugar assayed the concentration of soluble sugar , and 1 Introduction freezing tolerance in the nonacclimated and cold2ac2 climated seedlings. We also assayed the effect of Many plants increase freezing tolerance in re2 the extraneous saccharose2pretreatment on the con2 sponse to low temperatures , a process known as cold centration of soluble sugar and freezing tolerance of acclimation. Numerous cellular and metabolic seedlings to determine whether soluble sugar accu2 changes that occur during cold acclimation have mulation could affect the development of freezing ( been studied