低温纤维素降解菌的筛选及其混合发酵效果研究的中期报告.docxVIP

低温纤维素降解菌的筛选及其混合发酵效果研究的中期报告 摘要： 本研究旨在筛选一株具有高效低温纤维素降解能力的菌株，探索其在混合发酵中的应用效果。首先，从富含纤维素的土壤中分离出一株纤维素降解菌，经形态学、生理生化特性和分子生物学鉴定，其属于芽孢杆菌属。接着，以该菌株作为种子菌添加到含有纤维素底物的发酵液中，同时加入其他代谢产物合适的混合发酵菌，采用中压灭菌技术进行混合发酵，研究菌株的降解能力和代谢产物的生成情况。结果显示，所筛选的菌株具有较高的低温纤维素降解能力，在混合发酵中的应用效果也较为理想。最终，本研究为低温纤维素降解菌的筛选和混合发酵的应用提供了一定的理论和实践基础。 关键词：低温纤维素降解菌；混合发酵；代谢产物；筛选。 Abstract: The objective of this study is to screen a strain of cellulose degradation bacteria with efficient ability under low temperature and explore its application effects in mixed fermentation. Firstly, a cellulose degradation bacterium was isolated from the soil rich in cellulose. After morphological, physiological, biochemical characteristics and molecular biology identification, it belonged to Bacillus subtilis. Then, this strain was added as a seed bacterium to the fermentation liquid containing cellulose substrate, and a suitable mixed fermentation bacterium was added to generate other metabolic products. The mixed fermentation was carried out by mid-pressure sterilization technology to study the degradation ability of the strain and the generation of metabolic products. The results showed that the screened strain had high ability of low temperature cellulose degradation, and ideal application effects in mixed fermentation. Finally, this study provides a theoretical and practical basis for the screening and application of low temperature cellulose degradation bacteria in mixed fermentation. Keywords: low temperature cellulose degradation bacteria; mixed fermentation; metabolic products; screening.