荔枝AP1、CDPK同源基因的克隆及表达研究的中期报告.docxVIP

荔枝AP1、CDPK同源基因的克隆及表达研究的中期报告 荔枝AP1、CDPK同源基因的克隆及表达研究的中期报告 摘要： 本研究以荔枝为材料，从转录组测序中获得了两个同源基因序列，分别命名为LjAP1和LjCDPK。通过生物信息学方法分析LjAP1和LjCDPK的基本信息，包括长度、氨基酸序列、亲缘关系等。随后，采用PCR方法从荔枝果实中克隆出了完整的LjAP1和LjCDPK基因，进行了序列验证，并进行了进一步的分析。最后，利用实时定量PCR技术，对不同部位、不同时期的荔枝果实进行了LjAP1和LjCDPK基因表达量的检测。 初步结果表明，LjAP1和LjCDPK基因在荔枝果实发育过程中均表现出明显的不同表达模式。其中，LjAP1基因在果实生长和发育的早期表达较高，并随着果实成熟不断降低；而LjCDPK基因则在果实成熟后期表达较高，可能与果实的成熟和贮藏有关。此外，两个基因在果实不同部位也表现出不同的表达模式，这为下一步研究基因功能提供了重要的依据。 关键词：荔枝，AP1，CDPK，同源基因，克隆，表达分析 Abstract: In this study, we obtained two homologous gene sequences, named LjAP1 and LjCDPK, from lychee through transcriptome sequencing. The basic information of LjAP1 and LjCDPK, including length, amino acid sequence, and phylogenetic relationship, was analyzed by bioinformatics methods. Subsequently, the complete LjAP1 and LjCDPK genes were cloned from lychee fruit using PCR, and their sequences were verified and further analyzed. Finally, real-time quantitative PCR technology was used to detect the expression levels of LjAP1 and LjCDPK genes in different parts and at different stages of lychee fruit. Preliminary results showed that LjAP1 and LjCDPK genes exhibited different expression patterns during lychee fruit development. Specifically, LjAP1 gene was highly expressed in the early stage of fruit growth and development, and gradually decreased as the fruit ripened; while LjCDPK gene was highly expressed in the late stage of fruit ripening, which may be related to fruit maturation and storage. In addition, the two genes exhibited different expression patterns in different parts of the fruit, which provided important basis for the further study of gene functions. Keywords: Lychee, AP1, CDPK, homologous genes, cloning, expression analysis