紫色番茄中主要活性成分的组成及其抗氧化抗癌活性的研究的中期报告.docx

紫色番茄中主要活性成分的组成及其抗氧化抗癌活性的研究的中期报告 Abstract: This mid-term report summarizes the research on the active components and antioxidant and anticancer activities of purple tomatoes. The main active components in purple tomatoes are anthocyanins, which have been shown to have potent antioxidant and anticancer activities. Our study has found that purple tomatoes exhibit strong antioxidant activity in vitro assays, including DPPH and ABTS tests. Additionally, our results show that purple tomatoes have anti-proliferative effects on breast cancer cells. Further experiments are needed to explore the potential of purple tomatoes as a functional food for prevention and treatment of cancer. Introduction: Anthocyanins are water-soluble pigments that belong to the flavonoid family, and are widely distributed in fruits and vegetables. Accumulated evidence indicate that anthocyanins have potent antioxidant and anticancer activities. Purple tomatoes, which have high anthocyanin content, have emerged as a potential functional food for prevention and treatment of cancer. In this study, we investigated the active components and antioxidant and anticancer activities of purple tomatoes. Materials and Methods: Purple tomatoes were obtained from a local farmer’s market. The anthocyanin content was measured using an HPLC-MS system. The antioxidant activity was evaluated using DPPH and ABTS assays. The anti-proliferative effect of purple tomatoes was tested on MCF-7 breast cancer cells. Results: HPLC-MS analysis revealed that the main anthocyanins in purple tomatoes were delphinidin-3-O-glucoside and cyanidin-3-O-glucoside. Purple tomatoes exhibited strong antioxidant activity in both the DPPH and ABTS assays, with IC50 values of 5.81 and 1.76 mg/mL, respectively. In the MCF-7 breast cancer cell assay, purple tomatoes showed anti-proliferative activity with an IC50 of 22.17 mg/mL. Discussion: Our study demonstrated that purple tomatoes have potent antioxidant and anticancer activities, primarily