全反式维甲酸对UVB照射的A375细胞黑素代谢及cuznSOD的影响的中期报告.docxVIP

全反式维甲酸对UVB照射的A375细胞黑素代谢及cuznSOD的影响的中期报告 摘要： 本研究旨在探究全反式维甲酸对UVB照射的A375细胞黑素代谢及cuznSOD的影响。实验分为四组：对照组、UVB组、全反式维甲酸组和联合组。使用MTT试剂盒测定细胞存活率，ELISA法检测黑素生成酶（TYR）和酪氨酸酶（TRP-1）的表达水平，Western blot法检测cuznSOD蛋白的表达水平。结果显示，UVB照射可明显抑制A375细胞的存活，而全反式维甲酸预处理可增加A375细胞的存活率。同时，全反式维甲酸处理可显著降低UVB照射后A375细胞中TYR和TRP-1的表达水平，提示其对黑素合成有抑制作用；而全反式维甲酸与UVB联合处理组中TYR和TRP-1的表达水平则显著高于UVB组。此外，全反式维甲酸处理可显著增加A375细胞中cuznSOD蛋白的表达水平，而UVB照射后可显著降低cuznSOD蛋白的表达水平，联合处理组中cuznSOD蛋白的表达水平则显著高于UVB组。 关键词：全反式维甲酸；UVB照射；黑素代谢；cuznSOD Abstract: The aim of this study was to investigate the effects of all-trans retinoic acid on melanin metabolism and cuznSOD expression in A375 cells irradiated by UVB. The experiment was divided into 4 groups: control group, UVB group, all-trans retinoic acid group, and combination group. Cell viability was determined using MTT assay, while the expression levels of tyrosinase (TYR) and tyrosine-related protein-1 (TRP-1) were measured using ELISA method. The expression level of cuznSOD protein was detected by Western blot. The results showed that UVB irradiation significantly inhibited the survival of A375 cells, while pretreatment with all-trans retinoic acid increased the survival rate of A375 cells. Treatment with all-trans retinoic acid significantly reduced the expression levels of TYR and TRP-1 in A375 cells after UVB irradiation, indicating that it had an inhibitory effect on melanin synthesis. However, the expression levels of TYR and TRP-1 in the combination group were significantly higher than those in the UVB group. In addition, treatment with all-trans retinoic acid significantly increased the expression level of cuznSOD protein in A375 cells, while UVB irradiation significantly reduced the expression level of cuznSOD protein. The expression level of cuznSOD protein in the combination group was significantly higher than that in the UVB group. Keywords: all-trans retinoic acid; UVB irradiation; melanin metabolism; cuznSOD.