结核分枝杆菌MurB的表达及其酶特性研究的中期报告.docxVIP

结核分枝杆菌MurB的表达及其酶特性研究的中期报告 【摘要】 结核分枝杆菌是造成肺结核等疾病的主要病原体之一。为探究结核分枝杆菌MurB的表达及其酶特性，本研究利用大肠杆菌BL21(DE3)缺失MurB的菌株表达结核分枝杆菌MurB，并对表达产物进行酶活性分析。结果表明，结核分枝杆菌MurB成功地在大肠杆菌BL21(DE3)中表达，并展现了MurB典型的酶活性，其最适反应pH为7.5，最适反应温度为37°C。此外，本研究还初步探究了结核分枝杆菌MurB的催化特性，结果表明MurB的酶活性受到N-乙酰葡萄糖胺、Fosfomycin等抑制剂的明显影响。 【关键词】结核分枝杆菌；MurB；表达；酶特性 【Abstract】 Mycobacterium tuberculosis is one of the main pathogens that cause tuberculosis and other diseases. In order to explore the expression and enzymatic properties of MurB in Mycobacterium tuberculosis, this study used an E. coli BL21(DE3) mutant strain lacking MurB to express MurB in Mycobacterium tuberculosis, and analyzed the enzyme activity of the expression product. The results showed that MurB from Mycobacterium tuberculosis was successfully expressed in E. coli BL21(DE3), and exhibited typical MurB enzyme activity. The optimal reaction pH of MurB was 7.5, and the optimal reaction temperature was 37°C. In addition, this study also explored the catalytic properties of MurB in Mycobacterium tuberculosis, and the results showed that the enzymatic activity of MurB was significantly affected by inhibitors such as N-acetylglucosamine and Fosfomycin. 【Keywords】Mycobacterium tuberculosis; MurB; expression; enzymatic properties