酵母双杂交手册（英文）.pdfVIP

CLONTECH Innovative Tools to Accelerate Discovery Yeast Protocols Handbook PT3024-1 (PR13103) Published 14 March 2001 Catalog #:K1612-1 MATCHMAKER Two-Hybrid System 3 (many) MATCHMAKER GAL4 Libraries K1615-1 MATCHMAKER Library Construction Screening Kit K1603-1 MATCHMAKER One-Hybrid System NL4000AA MATCHMAKER Random Peptide Library 5398-1 GAL4 AD Monoclonal Antibody 5399-1 GAL4 DNA-BD Monoclonal Antibody FOR RESEARCH USE ONLY Yeast Protocols Handbook Table of Contents I. Introduction 4 II. Introduction to Yeast Promoters 5 III. Culturing and Handling Yeast 10 IV. Preparation of Yeast Protein Extracts 12 A. General Information 12 B. Preparation of Yeast Cultures for Protein Extraction 12 C. Preparation of Protein Extracts: Urea/SDS Method 13 D. Preparation of Protein Extracts: TCA Method 15 E. Troubleshooting