注射用氨苄西林钠、酚磺乙胺的体外配伍稳定性考察的开题报告.docxVIP

注射用氨苄西林钠、酚磺乙胺的体外配伍稳定性考察的开题报告

摘要

本文旨在探究注射用氨苄西林钠和酚磺乙胺的体外配伍稳定性，并提供实验数据为临床用药提供参考。文中使用UV‐vis吸收分光光度计、高效液相色谱仪(HPLC)和傅里叶变换红外光谱仪(FTIR)对氨苄西林钠和酚磺乙胺在不同条件下的混合溶液进行了检验。结果显示，氨苄西林钠和酚磺乙胺在pH值为4.0和7.4的水溶液中，配伍溶液颜色变化较小，峰面积和峰高变化不大，可以判定配伍稳定性较好。但当混合溶液在室温下保存72h后，HPLC检测出混合溶液中氨苄西林钠逐渐降解，加载量下降，且配伍稳定性降低。在紫外分光光度计检测下，发现氨苄西林钠在254nm处对光敏感，因此配伍溶液在室温下暴露于光线下72h后，药物活性下降，与配伍剂量呈现出较高的相关性。此外，使用傅里叶变换红外光谱仪进一步探究药物混合溶液的分子结构和化学键特征，结果表明，药物在混合溶液中确实发生了相互作用。本研究为氨苄西林钠和酚磺乙胺的联合用药提供了临床药学参考。

关键词：氨苄西林钠，酚磺乙胺，体外配伍稳定性，HPLC，UV‐vis吸收分光光度计，傅里叶变换红外光谱仪

Abstract

Theaimofthisstudywastoinvestigatetheinvitrocompatibilityofinjectableampicillinsodiumandphenobarbitalsodium,andprovideexperimentaldataforclinicalmedication.Inthisstudy,themixedsolutionsofampicillinsodiumandphenobarbitalsodiumunderdifferentconditionsweretestedusingUV-visspectrophotometer,highperformanceliquidchromatography(HPLC)andFouriertransforminfraredspectrometer(FTIR).TheresultsshowedthatthemixedsolutionsofampicillinsodiumandphenobarbitalsodiuminwaterwithpHof4.0and7.4hadminimalcolorchangesandlittlechangeinpeakareaandpeakheight,indicatinggoodcompatibility.However,after72hoursofstorageatroomtemperature,HPLCrevealedthatampicillinsodiuminthemixedsolutiongraduallydegradedandtheloadingdecreased,indicatingadecreaseincompatibility.UV-visspectrophotometryshowedthatampicillinsodiumwassensitivetolightat254nm.Therefore,afterexposuretolightfor72hoursatroomtemperature,thedrugactivitydecreased,andtherewasahighcorrelationwiththecompatibilitydose.Furthermore,FTIRwasusedtofurtherexplorethemolecularstructureandchemicalbondcharacteristicsofdrugmixedsolutions,andtheresultsshowedthatthedrugsdidinteractwitheachotherinthemixedsolution.Thisstudyprovidesclinicalpharmacologicalreferenceforthecombineduseofampicillinsodiumandphenobarbitalsodium.

Keywords:Ampicillinsodium,Phenobarbitalsod