分子生物学学习资料:MB answers 05.docVIP

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AnswerstoWeaverendofchapterquestions

Chapter5MolecularToolsforStudyingGenesandGeneActivity

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DNAloadedinwells

DNArunstowards

Thepositivepole

1200bp

600bp

150bp

SDS,sodiumdodecylsulfate,isadetergentusedinSDSpolyacrylamidegelelectrophoresis.SDSdenaturestheproteinsandseparatesthesubunitsofcomplexproteins.Inaddition,itcoatsallthepolypeptideswithanegativechargesothattheymigrateaccordingtotheirmolecularmassesandnottheirnaturalcharge.

SDSandmoderntwo-dimensionalgelelectrophoresisbothseparateproteinsaccord

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