分子生物学学习资料:MB answers 09.docVIP

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AnswerstoWeaverendofchapterquestions

Chapter9DNA-ProteinInteractionsinProkaryotes

SeeFigure9.2:

SeeFigures9.3and9.4.Aminoacidswappingexperimentscanbeusedtoshowwhichaminoacidsareimportantinspecifyingbindingofrepressorstotheiroperators.Intheseexperiments,aminoacidresiduesthatarepredictedbystructuralstudiestoplayaroleinbindingaparticularoperatorarealteredbysite-directedmutagenesis.Tousetheexamplegiveninthetextoftheλ-likephages434andP22,wewouldpredictthatifweweretoswaptheaminoacidswehypothesize

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