分子生物学学习资料:MB answers 06.docVIP

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AnswerstoWeaverendofchapterquestions

Chapter6TheMechanismofTranscriptioninProkaryotes

TheE.colicoreRNApolymeraseiscapableofbindingonlylooselyandnon-specificallytopromoters.ItwillbindT4phageDNAnon-specificallyandwilltranscribeitonlyweakly.Additionofthe??subunitwilladdspecificityandleadtotightbindingofRNApolymerasetoT4phageDNA,whichisrecognizedbyE.coliRNApolymerase.Interestingly,coreRNApolymerasetranscribeshighlynickedDNAtemplatesverywellbutthisisanartifact.Thisexplainstheefficienttranscriptionofa

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