加味当归芍药散方对DM早期肾损害大鼠血清SOD和MDA的影响的开题报告.docxVIP

加味当归芍药散方对DM早期肾损害大鼠血清SOD和MDA的影响的开题报告

【摘要】

本研究旨在探讨加味当归芍药散方对糖尿病早期肾损害大鼠血清超氧化物歧化酶（SOD）和丙二醛（MDA）的影响。选用60只雄性SD大鼠，随机分为正常组、糖尿病模型组、加味当归芍药散方治疗组和对照组。糖尿病模型组和加味当归芍药散方治疗组均采用链脲佐菌素（STZ）注射法建立糖尿病模型，其中加味当归芍药散方治疗组口服加味当归芍药散方。8周后，采集大鼠血清，测定SOD和MDA水平。结果显示，与正常组相比，糖尿病模型组SOD水平降低，MDA水平升高，差异均有统计学意义（P0.05）；与糖尿病模型组相比，加味当归芍药散方治疗组SOD水平升高，MDA水平降低，差异均有统计学意义（P0.05）；对照组与糖尿病模型组相比，未见明显差异。因此，加味当归芍药散方可能通过提高血清SOD水平、降低血清MDA水平发挥保护肾脏作用。

【关键词】加味当归芍药散方；糖尿病；肾损害；SOD；MDA

【Abstract】

ThepurposeofthisstudywastoinvestigatetheeffectsofJiaweiDangguiShaoyaopowderonserumlevelsofsuperoxidedismutase(SOD)andmalondialdehyde(MDA)inratswithearlydiabeticnephropathy.SixtymaleSDratswererandomlydividedintonormalgroup,diabeticmodelgroup,JiaweiDangguiShaoyaopowdertreatmentgroupandcontrolgroup.ThediabeticmodelgroupandJiaweiDangguiShaoyaopowdertreatmentgroupwereestablishedbyStreptozotocin(STZ)injection,andtheJiaweiDangguiShaoyaopowdertreatmentgroupwasgivenoralJiaweiDangguiShaoyaopowder.After8weeks,serumwascollectedfromratsandlevelsofSODandMDAweremeasured.Theresultsshowedthatcomparedwiththenormalgroup,theSODlevelinthediabeticmodelgroupdecreased,andtheMDAlevelincreased,thedifferencewasstatisticallysignificant(P0.05);comparedwiththediabeticmodelgroup,theSODlevelintheJiaweiDangguiShaoyaopowdertreatmentgroupincreased,andtheMDAleveldecrease,thedifferencewasstatisticallysignificant(P0.05);nosignificantdifferenceswereobservedbetweenthecontrolgroupandthediabeticmodelgroup.Therefore,JiaweiDangguiShaoyaopowdermayexertaprotectiveeffectonthekidneybyincreasingserumSODlevelsanddecreasingserumMDAlevels.

【Keywords】JiaweiDangguiShaoyaopowder；diabetes；nephropathy；SOD；MDA