Pt-Co3O4空心笼电催化水氧化超灵敏检测戊型肝炎病毒.pdf

SupportingInformation

UltrasensitiveDetectionofHepatitisEVirusby

ElectrocatalyticWaterOxidationusingPt-CoO

34

HollowCages

AkhileshBabuGanganboina,IndraMemdiKhoris,AnkanDuttaChowdhury,Tian-

ChengLi,EnochY.Park*

A.B.Ganganboina,A.D.Chowdhury,Prof.E.Y.Park

ResearchInstituteofGreenScienceandTechnology,ShizuokaUniversity,836Ohya

Suruga-ku,Shizuoka422-8529,Japan

I.M.Khoris,Prof.E.Y.Park

DepartmentofBioscience,GraduateSchoolofScienceandTechnology,Shizuoka

University,836OhyaSuruga-ku,Shizuoka422-8529,Japan

Dr.Tian-ChengLi

DepartmentofVirology2,NationalInstituteofInfectiousDiseases,4-7-1Gakuen,

Musashimurayam-shi,Tokyo208-0011,Japan

E-mailaddresses:

akhilesh.babu.ganganboina@shizuoka.ac.jp(ABG)

Indra.memdi.khoris.17@shizuoka.ac.jp(IMK)

ankan.dutta.chowdhury@shizuoka.ac.jp(ADC)

litc@nih.go.jp(TCL)

park.enoch@shizuoka.ac.jp(EYP)

S-1

PreparationofHEV-LPs,HEVandAnti-HEVIgG.TheG3HEV-LPswere

producedbyarecombinantbaculovirusexpressionsystemasdescribedpreviously.1

G7HEVswereproducedbycellculturewithahumanhepatocarcinomacellline,

PLC/PRF/5(JCRB0406,theHealthScienceResearchResourcesBank,Osaka,Japan).2

Atotalof1 mlofthe10%stoolsuspensionsamplewasinoculatedontoPLC/PRF/5

cells,andincubatedat36 °Cwith10 mlmaintenancemediumconsistingofmedium199

(Invitrogen,Carlsbad,CA)containing2%(v/v)heat-inactivatedFBSand10 mM

8−1

MgCl.TheRNAcopynumbersofG7,HEVswere5.0 × 10 copies mL.

2