类芽孢杆菌B69产胞外多糖絮凝剂优化、组成分析及其在污水处理中的应用的中期报告.docxVIP

类芽孢杆菌B69产胞外多糖絮凝剂优化、组成分析及其在污水处理中的应用的中期报告

Abstract:

Thismidtermreportconcernstheoptimizationoftheproductionofextracellularpolysaccharide(EPS)flocculantfromBacilluslicheniformisB69,theanalysisofitscomposition,anditsapplicationinwastewatertreatment.TheEPSflocculantwasproducedbycultivatingB.licheniformisB69inafermentationmediumcontainingglucoseandyeastextract.Responsesurfacemethodology(RSM)wasusedtooptimizethecultivationconditionstoimproveEPSproduction.TheEPSflocculantwasthenextractedandpurifiedbyethanolprecipitationanddialysis,anditscompositionwasanalyzedbyhigh-performanceliquidchromatography(HPLC)andgaschromatography-massspectrometry(GC-MS).TheEPSflocculantwasalsotestedforitsflocculationefficiencyintreatingsyntheticsewage.

Introduction:

BacilluslicheniformisisaGram-positive,spore-formingbacteriumwidelyfoundinsoilandplantmaterial.Ithasbeenreportedtoproducevariousbioactivecompounds,includingantibiotics,enzymes,biofilms,andpolysaccharides.Amongthem,extracellularpolysaccharides(EPS)areofgreatinterestduetotheirapplicationsinvariousfields,suchasfood,pharmaceuticals,andwastewatertreatment.EPSproducedbyBacillusspecieshavebeendemonstratedtopossessexcellentflocculatingproperties,whichcanbeutilizedtoremovesuspendedsolids,turbidity,andpollutantsinwastewater.

MaterialsandMethods:

Bacterialstrainandgrowthconditions:

Inthisstudy,weusedBacilluslicheniformisB69,whichwasisolatedfromsoilsamplesinourlaboratory,astheEPS-producingstrain.ThebacteriaweregrowninaLBmedium(10g/Ltryptone,5g/Lyeastextract,and10g/LNaCl)at37°C,withshakingat200rpmfor16hbeforeinoculation.

CultivationandEPSextraction:

TheEPSproductionmediumcontainedglucose(20g/L),yeastextract(5g/L),MgSO4·7H2O(0.5g/L),andKH2PO4(1g/L).Thecultivationwascarriedoutin250mLErlenm