水稻转化方法.docxVIP

水稻转化方法

水稻转化 (来自文献

/content/nt36q44450563406/fulltext.pdf)Forricetransformation,maturericeseedswerede-huskedandsterilisedwith3%sodiumhypochlorite(30min).TheseedswerethoroughlywashedthreetimeswithsteriledistilledwaterandtransferredontoMSbasalmedium(MurashigeandSkoog1962)containing500mg/lproline,300mg/lcaseinhydrolyte,2mg/l

dichlorphenoxyacidicacid(2,4-D)and30gsucrose,for1week.ThefreshlyinducedcalliweredippedinAgrobacteriumtumefaciens(AGL1strain)andtransferredontoNBmedium(N6macro-elements,B5micro-elements)containi