基于生物信息学分析SPP2在肝癌发生过程中的作用（英文）_NormalPdf.docxVIP

中南大学学报（医学版）

JCentSouthUniv(MedSci)

2023,48(12)

1779

ARTICLES··论著

DOI：10.11817/j.issn.1672-7347.2023.230077

SPP2playsaroleinthetumorigenesisofhepatocellularcarcinoma:Abioinformaticbasedanalysis

PENGHonghua1,LIUYang2,SONGZewen1

(1.DepartmentofOncology,ThirdXiangyaHospital,CentralSouthUniversity,Changsha410013;

2.DepartmentofPathology,ThirdXiangyaHospital,CentralSouthUniversity,Changsha410013,China)

ABSTRACT

Objective:Hepatocellularcarcinoma(HCC)patientsatthesamestageexhibitdifferent

prognosis,andtheunderlyingmolecularmechanismremainsunclear.Thisstudyaimsto

identifythekeygenesimpactingtheprognosisofHCCpatients.

Methods:DifferentiallyexpressedgeneanalyseswereperformedbetweenHCCsamples

andnormalones,andbetweenpatientswithlongoverallsurvival(OS)andthosewithshort

OS,inTCGA-LIHCandGSE14520datasets.TheKaplan-Meiermethodwithlog-ranktest

wasusedtoevaluatetheroleofsecretedphosphoprotein2(SPP2)intheprognosisofHCC

patients.Genesetenrichmentanalysis(GSEA)wasusedtounderstandthedifferenceof

enrichedsignalingpathwaysbetweenSPP2-stratifiedHCCsubgroups.GeneOntology

(GO)andKyotoEncyclopediaofGenesandGenomes(KEGG)analyseswereperformed

topredictthepotentialfunctionalpathwaysinwhichSPP2mightparticipate.

Results:SPP2wassignificantlydown-regulatedintumorswhencomparedwithnormal

tissues,orintumorsampleswithshortOSwhencomparedwiththosewithlongOS[fold

change(FC)2andfalsediscoveryrate(FDR)0.05].LowexpressionofSPP2was

associatedwithworseclinicopathologicalfeatureslikevascularinvasion(P=1.6e-05),poor

cancerstatus(withtumor,P=0.021),advancedTstage(T3orT4,P=4.5e-04),advanced

TNMstage(stageIIIorIV,P=3.1e-04),andwithunfavorableprognosis(